Use of Pseudomonas aeruginosa in the control od root-knot disease complex in tomato: the effects of different inoculum levels of Meloidogyne javanica and Rhizoctonia solani

Abstract

The potential impact of Pseudomons aeruginosa strain IE-6 as a biological control agent against Meloidogyne javanica at four inoculum densities (0, 250, 500 and 1000 eggs/plant) and Rhizoctonia solani at three inoeulum levels (0, 1 and 3 ml culture suspension/kg of soil) was examined on tomato in the greenhouse experiments. The biocontrol bacterium suppressed root infection caused by R. solani and M. javanica on tomato in both sterilized and non-sterilized soils. Root-rot infection increased with the increase in pathogen(s) concentration. P. aeruginosa showed better biocontrol effects at low population levels of M.javanica and R. solani than at higher population densities of the pathogen(s). Root-rot disease severity was more pronounced in sterilized soil compared to the non-sterilized one. Soil infested with high population densities ofR. solani (3 ml /kg of soil) and M. javanica (2000 eggs/pot) resulted in complete mortality of tomato seedlings in sterilized soil, whereas some plants were found to survive in non-stenlized soil. There seems to be a correlation between population density of M. javanica and root colonization by R. solani. Root colonization by other three root-infecting fungi including Macrophomina phaseolina, Fusarium oxysporum and Fusarium solani was also lower in the presence of P. aeruginosa in non-sterilized soil. P. aeruginosa enhanced plant growth in both types of soil.