Effect of Botrytis cinerea infection and elicitation on ß-1,3-glucanase and chitinase activity in bean leaves and cell cultures

Elżbieta Kuźniak, Henryk Urbanek, Aneta Michalak, Katarzyna Herka


The activity of ß-1,3-glucanase and chitinase in bean plants treated with B. cinerea products or/and infected and in cell cultures after application of fungal products has been studied. Botrytis cinerea infection and culture filtrates, ethanol precipitates, glucan and conidial extract treatment markedly enhanced the activity of both hydrolases. Cell cultures treated with B.cinerea products reacted similarly to intact plants. In plants pretreated with 2-day culture filtrate and conidial extract and then infected, ß-1,3-glucanase and chitinase were induced stronger than after infection without pretreatment.

Full Text: