The elimination of viruses from garlic (Allium sativum L.) plants by thermotherapy and meristem tip culture

Marek S. Szyndel, Bożena Kozera, Ewa Misterek

Abstract


The production of virus free garlic plants from totally cvs Jana, Mera and ecotype Zamojski was attempted by means of thermotherapy and meristem tip culture. The cloves and the aerial bulbils after hot air treatment in a growth chamber at 36ºC for 30-35 days or at 26-28ºC for 3-4 months in greenhouse were used to meristem tip culture on M. S. medium. In the 26-28ºC treatment 19.5 % of meristerns produced plants and 22.5 % of these were virus free. In the 36ºC treatment 14.5 % of the meristems developed into plantlets and 34.6 % of them were virus free. The plantlets were indexed by "sap-dip" electron microscopy methods.

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DOI: https://doi.org/10.5586/aa.1994.005

Journal ISSN:
  • 2300-357X (online)
  • 0065-0951 (print; ceased since 2016)
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Publisher
Polish Botanical Society