Haemolytic micromethod for rapid estimation of toxic alfalfa saponin

Marian Jurzysta

Abstract


On a plate covered with a thin layer of blood suspension with gelatine 10 microliters of alfalfa juice or alfalfa meal extract is spotted. As a result of saponin diffusion and their reaction with blood a haemolytic ring appears, the diameter of which is proportional to the concentration of toxic saponins. Selected by this method low-saponin individual alfalfa plants proved non toxic for fishes and did not inhibit the growth of Trichoderma viride.

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DOI: https://doi.org/10.5586/aa.1979.001

Journal ISSN:
  • 2300-357X (online)
  • 0065-0951 (print; ceased since 2016)
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Publisher
Polish Botanical Society