The effect of methyl jasmonate on ethylene production and CO2 evolution in Jonagold apples

Artur Miszczak, Edward Lange, Marian Saniewski, Janusz Czapski

Abstract


Apples cv. Jonagold were harvested at the beginning of October and stored at 0°C until treatment between the beginning of December and the end of January. Methyl jasmonate (JA-Me) at the concentration of l,0, 0,5, 0,1, 0,05, and 0,01% in lanolin paste were applied to the surface ofintact apples. During five days from treatment, samples of cortex with skin (area about 2,0 cm2) were cut off at a depth of about 2 mm and used for determination of ethylene production, ACC oxidase activity and respiration determined as CO2 evolution. The production of endogenous ethylene was highest at mid-January ( 100, 280, and 250 nl/g*h at December, mid-January, and the end of January, respectively). During December and at the beginning of January, JA-Me initially ( 1 -2 days after treatment) stimulated ethylene production and then the production was inhibited. The lower concentration of JA-Me caused initially the greater stimulation and then Iower inhibition of ethylene production. However, at the time of maximum production of endogenous ethylene (mid-January) and later. stimulatory effect of JA-Me disappeared. The effect of various concentrations and time of application of JA-Me on ACC oxidase activity had similar trend as endogenous ethylene production. Methyl jasmonate stimulated respiration and this effect was dependent on JA-Me concentration and independent on time of application. The metabolic significance of these findings is discussed.

Keywords


apples; methyl jasmonate; ethylene; ACC oxidase; respiration

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