Zastosowanie odmian Raphanus sativus do oznaczania aktywności cytokinin testem biologicznym [The use of cultivars of Raphanus sativus for cytokinin bioassay]

Dorota Kubowicz, Ewa Grodzka

Abstract


Six cultivars of radish (Raphanus sativus) were tested for their usefulness in radish cytokinin bioassay by the method of Letham (1971). The best cultivar was found to be 'Sopel Lodu' which responds well to both zeatin and 2iP over a wide range of concentrations. The fresh weight of cotyledons increased at most by 71.5% (if treated with zeatin) or 101.0% (if treated with 2iP) compared to untreated cotyledons. This cultivar is also sensitive to the partially purified cytokinin-like fraction isolated from the pine (Pinus silvestris) cambial region. The cultivar 'Sopel Lodu' is therefore proposed to be a suitable plant for cytokinin bioassays.

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