High-performance liquid chromatography for determination of α-tocopherol in vegetables

Marcin Horbowicz


A simple method for the determination of α-tocopherol in vegetables is described. The procedure consists of the following steps: saponification, extraction, silica-column clean-up, and high-performance liquid chromatography. Elution time for D, L-α-tocopherol was 9.0 min using a Zorbax Sil (250 x 4.6 mm) column and an isocratic mobile phase of hexane-methanol (99.3 + 0.7), with a flow rate of 1 ml/min, and detection at 292 nm using a variable UV detector. The average recovery of α-tocopherol was 91.2%, and the minimum detectable amount was 0.1 mg/100 g of fresh vegetable tissue. This method is comparable to gas-chromatographic determination of α-tocopherol, but has fewer analytical steps and gives more reproducible results.

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DOI: https://doi.org/10.5586/aa.1989.018

Journal ISSN:
  • 2300-357X (online)
  • 0065-0951 (print; ceased since 2016)
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