In vitro micropropagation of chive (Allium schoenoprasum L.)

Marek Lubomski


The investigations on micropropagation of Allium schoenoprasum embraced shoot formation from cultured shoot tips, shoot multiplication, root formations, and cold storage of rooted plants. All explants were cultured on modified Murashige-Skoog medium. Surface disinfection of shoots derived from stock plants was not necessary to obtain no infection culture. The highest shoot multiplication was obtained on medium with 20 g l-1 sucrose and 1.0 mg 1-1 6-benzylo-arninopurine (BA). Root formation was observed in both indoleacetic acid (IAA) and indolebutyric acid (IBA). Rooted plants were successfully kept in cold storage (5°C ± 1°C darkness). Only 20% of plants died after 6 months of storage.

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