Zasiedlenie kostrzewy trzcinowej (Festuca arundinacea Schreb.) przez Neotyphodium coenophialum oraz jego wpływ na rozwój wybranych mikroorganizmów in vitro [Infestation of tall fescue (Festuca arundinacea Schreb.) with Neotyphodium coenophialum and its influence on growth of chosen microorganisms in vitro]

Abstract

Occurrence of Neotyphodium coenophialum in tall fescue cultivars cultivated in Poland and determination an endophyte inhibition effect on mycelium growth of chosen microorganisms in vitro were investigated. Seventeen seed lots of 11 cultivars of tall fescue were examined. The endophyte mycelium was dyed with bengal rose and microscopically examined to detect N. coenophialum. Occurrence of endophyte was checked with PCR method. Influence of endophyte on growth of 15 microorganisms was established in the laboratory conditions on Petri dishes with PDA medium at 10, 20 and 30^°C. Neotyphodium coenophialum occurred only in two seed lots, 'Barrocco' - 42% and Terros - 2%. Living mycelium of endophyte was isolated only from 'Barrocco'. The highest mycelium growth inhibition of Bipolaris sorokiniana, Fusarium avenaceum, F. equiseti, Microdochium nivale and Gaeumannomyces graminis by endophyte at 30^°C was recorded. The highest width of growth inhibition zone (4mm) was detected for the last pathogen. Mycelium growth of B. sorokiniana and M. nivale was not inhibited at 10^°C, and for F. avenaceum at 10 and 20^°C.